Supplementation of Buffalo Follicular Fluid: Beware of Other Sources of Steroid Hormones during Culture
نویسندگان
چکیده
An attempt was made to describe the updated information about the factors to be concerned before using of buffalo follicular fluid for supplementation in vitro of buffalo oocyte. There are not only diameter of the follicle, the present of corpus luteum (CL), growth of follicles, stage of estrus cycle, healthy and atretic state of the ovarian follicles. The progesterone and estradiol-17B concentration of follicular fluid from individual laboratory have to be determined for optimization of follicular fluid for supplementation. Fortunately, the effect is not severe because of the mixture of fluid from various stages of oestrous cycle. It is suggested to pre -determine the progesterone and estradiol-17β concentration of follicular fluid from different size of follicles and the present of CL, whose ovaries, retrieved from slaughterhouse, were at unknown estrus cycle. The other natural sources of progesterone and estradiol-17β were also discussed in this study. Therefore, the amount of supplementation may be not as high as expected. In conclusion, optimization of follicular fluid is suggested. This might be lead to reduce the amount of chemical steroids supplementation. Hence, the cost of expense is reduced.
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